EOSCARE IMMUNOASSAY ANALYZER

It’s ground-breaking, thorough, and essential for quantitative exams. It is an autonomous in-vitro diagnostic equipment that quantitatively evaluates the concentration of analytes in blood or other samples.

Sensitive

TRFIA is a time-resolved fluorescence immunoassay that combines extended decay lifetime markers with time-resolved fluorescence to effectively remove non-specific fluorescence interference while also considerably boosting analytical sensitivity.

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Establish assay critical success factors (i.e. sensitivity required).

2.

Ensure appropriate antibody and antigen reagents are available.

3.

Adsorb antigen or capture antibody to a solid surface.

4.

Wash off unbound reagents.

5.

Block nonspecific binding sites to reduce background.

6.

Incubate the secondary antibody with the sample.

7.

Wash off unbound reagents.

8.

Incubate secondary antibody-conjugate with sample.

9.

Wash off unbound reagents.

10.

Incubate substrate to generate signal.

11.

Calibration curve fitting, data analysis and quantitation by non-linear regression.

Develop and validate a method for incubation of compounds with a relevant target in the screening mode.

6.

Develop a sample collection procedure from screening experiments.

7.

Develop data analysis procedures to use immunoassay data to derive compound potency such as IC50 or EC50.